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C57BL6/J (2-3 months old) and C57BL/6J 22-244 moldh old) male mice were obtained from pathogen free breeding colonies at The Jackson Laboratories and NIH respectively.
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Female CD1 mice were obtained from the specific pathogen-free breeding facility at the University of Auckland.
Elimination of the parasite after birth by chemotherapy may provide negative off-spring and thus N. caninum-free breeding lines.
The first attempts of semi-free breeding of foxes were undertaken by American trappers in the 18th century.
Young (2 3 months), C57-BL/6 mice mice were obtained from pathogen-free breeding colonies at Jackson Laboratories (Bar Harbor, ME, USA).
Wistar rats were maintained in our own animal facility and were fed ad libitum with a soy-free breeding diet (SDS, Dundee, Scotland).
Furthermore, as the milk yield and gain of body weight appeared to be reduced in persistently infected cows [ 18], creation of Neospora-free breeding lines may also improve other economic aspects.
Wistar rats were maintained in our own animal facility according to UK Home Office guidelines [ Animal Scientificc Procedures) Act 1986], and were fed a soy-free breeding diet (SDS, Dundee, Scotland).
Mice were bred in our Bordetella- and pathogen-free breeding rooms at The Pennsylvania State University, and all animal experiments were performed with approval and in accordance to institutional guidelines.
While the future trends in Adélie penguin abundance remain uncertain, our study suggests that ice sheet retreat and the availability of ice-free breeding areas may be overriding factors in determining the millennial scale abundance trends of this species.
Our study has shown that East Antarctic Adélie penguins responded similarly to Scotia Arc Adélie penguins during climate warming following the LGM, with both populations undergoing an increase in population size coincident with an expansion of ice-free breeding habitat.
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