Exact(1)
Pt NN was prepared by template free borohydride reduction.
Similar(59)
After fixation, specimens were washed for 10 min on ice in PB/AA, treated for 30 min with 0.5% sodium borohydride in PB/AA to reduce free aldehyde groups, and washed again for 10 min in PB/AA.
Free-floating slices were quenched in sodium borohydride (0.5% in PBS) for 10 minutes and rinsed 3 times with PBS.
To block endogenous peroxidase activity the sections were incubated with 6% hydrogen peroxide (15 min at RT). Sodium borohydride (10 mg/ml PBS) was used to quench the free aldehyde groups (15 min).
Finally, any free aldehydes were blocked by 3 × 5 min incubations with 1 mg/ml sodium borohydride.
Support-free interconnected Pt nanowire networks (Pt NWNs) were fabricated by rapidly pouring sodium borohydride (NaBH4) solution into the Pt precursor solution, where hydrogen bubbles were in situ generated from hydrolysis and oxidation of NaBH4 as a dynamic template.
Free aldehyde groups on the slides were then quenched for 5 min in a sodium borohydride solution (3:1 PBS/EtOH 2.5% NaBH4).
For the immunohistochemical localization of GSK3β and phospho-serine-9 GSK3β (pSer9GSK3β) free-floating sections were rinsed in phosphate buffered saline (PBS), quenched in 1% sodium borohydride in PBS for 15 minutes, rinsed multiples times in PBS, and the endogenous peroxide was blocked in a solution of 1.5% hydrogen peroxide in PBS for five minutes.
Suitable reducing agents preferably sodium borohydride is used to yield the sodium salt of FEX which is converted to the free base by pH adjustment.
Free-floating coronal sections at the level of the mPFC (30 μm) were incubated in 0.5% sodium borohydride to reduce autofluorescence.
Magnesium borohydride (Mg(BH4 2, 95%) lithium borohydride (LiBH4, 95%), anhydrous tetrahydrofuran (THF), magnesium ribbon (99.5%) were purchased from Sigma Aldrich.
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