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Because some bacteria may thrive only as animal or plant parasites or only in a rich source of nutrients such as milk, they likely do not thrive as free bacteria in nature.
Also, in free bacteria sample, 1 ml of free bacteria suspension harvested from simulated intestinal juice was used for cell count by "pour plate count method" (see "Enumeration of free and microencapsulated probiotic bacteria").
In free bacteria samples, after centrifugation, 1 ml distilled water added to the tubes (Ghorbani-Choboghlo et al. 2015; Pourjafar et al. 2016).
The number of microencapsulated bacteria with 10%, 5% GT was significantly higher (P < 0.05) than microencapsulated bacteria without GT and free bacteria.
Two groups were designed as follows: One group was the whole bacteria which were labeled using fluorescence through removing free bacteria from rumen fluid (WFLB); the other group was the bacteria which were labeled using fluorescence without removing free bacteria from rumen fluid (FLB).
Subsequent to incubation, aliquots of 1 g of single coated beads or 1 g of double coated beads or 1 ml of free bacteria suspensions harvested from simulated gastric juice were added to 9 ml of sterilized simulated intestinal juice (0.05 mol l−1 KH2pH4, pH 7.5, with 1 g 100 ml−1 bile salt).
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The reduction rate of this free bacterium's count was 6.5-log (Additional file 1: Table S1).
Dr Venter has offered his invention for patenting an action that is sure to be controversial and the watermark will thus stake out what he hopes will become the property of his firm, Synthetic Genomics.Once the finished genome was inserted into the genome-free bacteria, the work regressed to the sort of microbiology that would have been familiar to the science's 19th-century pioneers.
By contrast, at a given value of the transmembrane potential, a CMA3 peptide-complexed bacteria which would display a net surface charge deficit associates faster with the α-HL nanopore at the lumen entrance, while the overall voltage-dependent association follows the same tendency as for the peptide-free bacteria.
Since plasmid-bearing bacteria have a longer generation time than plasmid-free bacteria, we assumed a 40% increase in generation time among the plasmid-bearing bacteria [36], [44].
SEM analysis of strains showed cells embedded completely in an extracellular matrix in the case of sessile samples collected from the opaque variant (Fig. 3A), whereas for sessile samples generated by the transparent variants, only matrix-free bacteria were observed (Fig. 3B).
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