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With increasing log kill, an increasing proportion of modelled patients with sensitive tumours is rendered relapse free, as indicated by the progressive shift in the notch point in the distribution to the left.

Expression analysis of samples prior to the additional DNase treatment showed the RT reactions conducted with samples extracted via the Maxwell® and SimplyRNA HT kits were gDNA free (as indicated by Ct values >35, Table  4) suggesting that the DNA removal procedures utilized by these kits (i.e., DNase I treatment) is effective for the tissue types evaluated here.

All samples of RNA were protein free as indicated by absorbance ratio 260/280 nm of 1.8 2.1.

Different types of bioactive compounds present in PTVO might have donated an extra electron to neutralize the effects of the DPPH free radical as indicated by the change in color of the reaction medium from dark purple to yellow [45].

Cells were incubated in serum-free media (Opti-MEM) ± test drugs as indicated and then washed twice with opti-MEM or as indicated.

The resulting network is a scale free network, as indicated by the distribution of connectivity that follows a power law distribution which is indicative for a scale free network (Additional file 5: Figure S1) [ 32].

However, the drying shrinkage of the samples is significantly increased with the increase of LP, caused by the more free water as indicated by the mass loss of the samples.

Open image in new window Fig. 6 Morphology of the equilibrium crystal shape nano-particles of CeO2 (a) and c-ZrO2 (b) obtained by the minimization of the Gibbs free energy, as indicated by the Gibbs-Wulff theorem.

By minimization of the Gibbs free energy as indicated by the Gibbs Wulff theorem, it was possible then to calculate the equilibrium crystal shape of a CeO2 nano-particles and show that under oxygen-rich conditions, the (111) surface is the only orientation present in the particle and the resulting morphology is described as the octahedron shown in Fig. 6a.

Peptides were added to NRCM cultured in serum free media, as indicated.

In particular, G3.5 dendrimers permeated the rat jejunum very well compared to free FITC, as indicated by a Papp in excess of 7 × 10 6 cm/s.