The Caesar salad garnished with anchovy was fine, but only the mussels, the frisée and pistachios were praiseworthy in the $15 poached seafood salad, which also included nearly flavor-free shrimp, scallops and calamari.
These results suggest that this assay is applicable widely as a new quantitative detection method in the pursuit of YHV-free shrimp culture.
The antiserum reacted with purified recombinant PmLamr and crude muscle tissue proteins from both P. monodon and P. vannamei, but not with hemocyte-free shrimp hemolymph.
PCR amplification with the primers generated a product of the expected size from the purified IHHNV DNA of Litopenaeus vannamei and IHHNV-infected penaeid populations but not from the IHHNV-free shrimp, white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV).
Nevertheless, the developed method remains useful for selection of specific pathogen-free shrimp.
Then the virus-free shrimp were infected with WSSV at 10 virions per ml by intramuscular injection using a syringe with a 29-gauge needle [ 46].
In addition, previous reports demonstrated that P. vannamei and P. stylirostris with pre-infection of IHHNV were more resistant to white spot syndrome virus (WSSV) as compared to the IHHNV-free shrimp [ 12, 13], although the mechanism remains unknown.
Our data showed that the primers and TaqMan probe were specific for HPV, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis virus (IHHNV), white spot syndrome virus (WSSV) and specific pathogen free (SPF) shrimp DNA.
