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Additionally, an imaging strategy called Fermi photoactivation was proposed, which improves the temporal separation in the activation of different molecules, thus helping to overcome undercounting due to the overlapping of molecules in the initial frames of imaging, which might occur when using a fixed activation power during the whole imaging time (Lee et al. 2012).
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By averaging the training data over several frames of the imaging the signal to noise ratio was effectively increased.
We also observed cell death in approximately 31% of cells expressing GFP PERP and MDM2 YFP over the time frame of imaging, which corresponded to approximately 30 48-h 30 48-hansfection (PT).
For all other parameters, the values given here are applied for all our movies, which include different cell types, frame rate, and magnification of imaging.
Advanced localization analysis algorithms detect higher densities of molecules per frame [55,56], giving a prospect of imaging fast repair events and chromosome conformations in a series of reconstructed images.
Conventional STED increases the resolution but damages hemozoin and any surrounding cellular structures as evidenced in confocal images recorded after a single frame of STED imaging.
With the frame trigger, the frame rate of imaging can be controlled together with adjusting the line density of OCT scanning.
Perfusion-like images from early time frames of PET imaging are more suitable for image co-registration with morphological MRI [6, 7].
a, Schematic of imaging approach.
Furthermore, UF-CBCT imaging achieved a reduction of radiation dose due to reduction of total number of acquired frames during imaging.
Outgrowth was measured as the displacement in μm of the distal tip of the growth cone between the first and last frames of an imaging session divided by the duration of that session.
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