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Prediction analysis, suggested that the protein product containing S168F was of normal length (599 amino acids) and that the correct reading frame was maintained.
One knock-in was incomplete and produced a frameshift; the other had an extra insertion after the 2×FLAG tag, but frame was maintained.
The nucleotide sequences for each cluster were aligned with PAL2NAL v.14 [ 155] to correspond to the trimmed amino acid alignment and ensure that the correct reading frame was maintained.
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The normal reading frame is maintained outside these nucleotide changes.
Subsequently, the queen was removed and the eggs contained in the frame were maintained in the honeybee colony for further development.
In order to detect only alternative splice events in which the correct reading frame is maintained, the phase of both exons joined by the alternatively spliced junction was calculated and only those junctions with exons of compatible phase were entered into the database.
Despite these repeats, the original reading frame is maintained, suggesting that the genes are functional.
After releasing the queen from the cage, the eggs contained in the frame were maintained in the honey bee colony for further development.
On the basis of analysis of the zebrafish nebulin sequence, the nebulin open reading frame is maintained with loss of exon 43.
Insertion of this gene trap cassette results in GFP expression if the open reading frame is maintained in the spliced transcript.
There are no translational termination codons in the inserted sequence, and since the WWOX translational reading frame is maintained, the transcript can potentially encode an approximately 30 kDa protein.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com