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Besides assessing the specificity of each outcome, the presence or absence of a time frame was collected for every outcome.
One frame was collected every 2 s.
At the end of the experiment, a region free of cells was selected, and one averaged background frame was collected at each excitation wavelength for background correction.
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Along each genome reference sequence the first 36 bp (or 75 bp) and the reverse complement of the last 36 bp (or 75 bp) in each window frame were collected than add an error rate of 0.1% to the reads.
Series of 500 frames (128 × 64 pixels per frame) were collected at a scanning frequency of 25 ms per frame to monitor fluorescence changes over time.
Before photobleaching, 10 frames were collected as an internal control of the experiment.
For each imaging session, the sequence was repeated five times and a total 800 calcium imaging frames were collected.
Here, rather than taking a single individual image of each area of the sample, a full sequence of individual "movie" frames is collected.
The image and motion features of patches extracted from each key-frame are collected and used to train an appearance motion codebook.
Eighty-three complete sets of the four frames were collected and then analyzed using a paradigmatic approach typical of qualitative content analysis to explore commonalities among the teachers' experiences.
Film projectors flash 48 images per second (24 frames are collected each second, but each frame is flashed twice) and high-end digital projectors can flash even more, according to Thomson researchers.
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