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eGFP gene was inserted into the viral BXLF1 open reading frame to monitor the activation of EBV and the infection of ECs.
Series of 500 frames (128 × 64 pixels per frame) were collected at a scanning frequency of 25 ms per frame to monitor fluorescence changes over time.
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This was followed by continuous imaging of the Cy3-RNA channel (561 nm excitation, 236 ms capture time [200 ms exposure +36 ms image acquisition]) for 700 frames to monitor the behaviour of RNA-motor complexes.
Series of 500 frames sampled at 98 ms per frame (128 × 128 pixels per frame) were used initially to monitor fluorescence changes over time.
The vectors include six stop codons upstream of ATG- GA 50 (two in eATG- GA 50g frame) twoprevent ATG-initiateachreadingion frametoe vector sequence, and a different tag in each reading frame downstream of ATG-(GA)50 to monitor prevent expression in all frames [i.e., frame 1: (GA)50-HA; frame 2: novel RAN protranslationrame 3: novel RAN protein-from] (Online Resource 4a).
"We can learn from our experiences with the consent decrees on how to frame them and how to monitor them," said Richard Blumenthal, the attorney general in Connecticut.
Clear time frames will also enable you to monitor time and effort taken into the hiring process.
Additional connectors allow to monitor the frames transferred on both channels (AirData and AirFT signals) and some interface signals, e.g. for the USB port, on a logic analyser for debug purposes.
The UHS Scheimpflug camera takes over 4,300 frames per second in order to monitor corneal response to a metered, collimated air pulse with symmetrical fixed profile and a fixed maximal internal pump pressure of 25 kPa.
The task of the participant was to monitor the stream of frames for a change in color of the fixation marker (randomly chosen frames were changed from black and white to red and green).
A near-infrared (NIR) probe was installed into a Fette 3090 feed frame to understand and monitor the die filling process.
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