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Square licenses the ally training from Frame Shift Consulting.
The frame shift at 408 has never been described before.
Better parameter sets (feature vector, number of frame shift, etc).
No stop codons or frame shift mutations were observed in the alignment.
We find that Apn1 expression does not affect frame shift mutation rates (Fig. 3).
The BdID1 genomic sequence had a 1 bp insertion giving a frame shift mutation.
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"fs" is for frame-shift.
Plasmids pCMVflagUL76-77HA, pCMVflag-frame-shift-UL76-77HA, pCMVflagUL76-77-frame-shift-HA pCMVflagUL76-77-frame-shift-HA pCMVflagUL76-77-frame-shift-HA pCMVflagUL76-77-frame-shift-HA pCMVflagUL76-77-frame-shift-HA
We also used this method to reveal the possible frame-shift sites and missing region.
Exonic INDELs were sorted into eight categories: frame-shift deletions, frame-shift insertions, frame-shift block substitutions, non-frame-shift deletions, non-frame-shift insertions, non-frame-shift block substitutions, stop-gains and stop-losses (Additional file 6).
Transcripts with a frame-shift were discarded.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com