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However, the measurements of some local estimates in this scheme are not always available due to intracoded frame prediction.
The approach implements the hierarchical B frame prediction implemented in the H.264/AVC JM 17.2 reference software encoder, and it is tested using the main and the stereo high profile.
One may involve the use of more sophisticated source encoding strategies, such as hierarchical bi-directional prediction (B-pictures) and long-term frame prediction with pulsed quality, which are more efficient but will introduce additional source coding delay.
The multi-view video coding (MVC) extension of the H.264/MPEG-4 AVC standard [5] exploits inter-view dependency in a simple, yet effective ways; images from other views (but at the same time instant) can be used as references for the current frame prediction (inter-view prediction).
The approach implements the hierarchical B frame prediction implemented in the H.264/AVC JM 17.2 reference software encoder, and it is tested using the Main and the Stereo High Profile, as well as, two of the most well-known search algorithms available in this reference software.
In assembly #4, unique sequences (UniSeqs) were subjected to open reading frame prediction by ESTScan [33], and we identified short-sequence-repeat microsatellites and sequence variations using Sputnik (http://espressosoftware.com/pages/sputnik.jsp) and local algorithms [30] respectively.
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The genome sequence and open reading frame predictions for A. fumigatus Af293 were obtained from GenBank accession numbers NC_007194 to NC_007201 (chromosomes 1 to 8).
Open reading frame predictions were carried out with the Glimmer3 program [ 51] assigning the "lpa" locus tag to each sequence.
As additional verification, the rice main open reading frame predictions were also compared with protein data from The UniProt Knowledgebase (UniProtKB).
Human testis total RNA was obtained commercially from AMS Biotechnology Ltd. (Cat. No. R1234260-50), and used to synthesize cDNA using a SMART RACE kit (Clontech Laboratories Inc .. Based on open reading frame predictions made from genomic DNA, gene-specific primers were used to amplify and clone ARGFX cDNA covering the complete predicted open reading.
To our knowledge this is the first time that substantial meta-information including quality values, open reading frame predictions and homology to genes in Arabidopsis thaliana, has been used on tomato sequences to perform a pre-genomic analysis of gene variability and evolution.
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