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Within the mitochondrial genome, a large repeat structure consisting of ~9 kb was found along with a novel 457 amino acid open reading frame of unknown function.
In this strain, the streptomycin resistance aadA2 gene cassette in one of the SGI1 integrons was replaced by a dfrA1 gene cassette, conferring resistance to trimethoprim and an open reading frame of unknown function.
The 3´-conserved region contains either a qacEΔ1 gene that encodes resistance to quaternary ammonium compounds or a combination of 3 genes: qacEΔ1, sulI (which encodes resistance to sulfonamides), and orf5 (an open reading frame of unknown function).
Toxigenic V. cholerae have been shown to contain a compound transposoon-like structure, the CTX genetic element which comprises a core region that contains the cholera toxin A and B subunits (ctxAB), zonular occludens toxin (zot), accessory colonization enterotoxin (ace), an open-reading frame of unknown function (orfU) and core-ended pilus (cep) genes.
The 3′-CS consists of the qacEΔ1 gene, which encodes an incomplete version of a protein that mediates the resistance to certain detergents, the sul1 gene, which encodes resistance to sulphonamides, and an open reading frame of unknown function orf5 [ 23].
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The korAB genes were followed by two open reading frames of unknown function (orf3 and orf4).
The tag to gene mapping was performed using SAGE Genie downloads and the tag to gene classifications of the 1,121 transcript tags shows that 80% of the tags can be mapped to well-defined transcripts, 5% match to hypothetical proteins/open reading frames of unknown function and 13% represent unknown transcripts (Figure 2B).
These missing sections included an intronic endonuclease and two downstream open reading frames of unknown function.
Plant mitochondrial genomes are notorious for their large and variable size, nonconserved open reading frames of unknown function, and high rates of rearrangement.
Similarly, N. oceanica and N. oculata encode two unique, small reading frames of unknown function not found in N. salina or N. gaditana.
CD, conserved domain; CFB, Cytophaga-Flavobacteria-Bacteroides; Indel, insert or deletion; ORF, open reading frame; ORFans, open reading frames of unknown functions; AlaRS, alanyl-tRNA synthetase; RGC, rare genetic change; RpoC, RNA polymerase β '-subunit.
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