Direct tests of this hypothesis can now be framed as predictions concerning epigenetic modifications of expression of the Protocadherin11XY gene pair either through methylation of DNA sequences, in part excluded by Ross et al. [ 2003], or modifications of other elements of epigenetic control at the level of histone structure.
To our initial surprise, despite using expression constructs that provided a common promoter, 5' UTR, N-terminal epitope tag and 3' UTR, and that differed only by the open reading frame (ORF), the levels of expression of nPTB and ROD1 in a range of cell lines were only 1 3% those of PTB.
This may be due to the slight differences in the time frame of expression and nuclear localization of CycE between the KLN-205 cells and the SCC.
The recombinant protein in frame of the expression vector pET28a were expressed and purified successfully.
Fatty-acid oxidation was accommodated by the identical time frame of the upregulation of the expression of TCA-cycle enzymes and the proteins of the electron-transport chain in response to fasting.
To confirm accurate insertion into the frame of the expression vector, sequencing chemistry were performed.
In cases where mCherry fusion occurs out of frame, the expression of fusion products can be unmasked by in situ hybridization with antisense mCherry probes.
The T2A sequence causes ribosomal 'skipping' (Donnelly et al., 2001), generating two proteins from a single open reading frame and resulting in stoichiometric expression of the gene of interest and the fluorescent reporter (Tang et al., 2009).
Using a random lacZ insertion method, Burns et al. and Ross-Macdonald et al. revealed extensive translation of short and out of frame ORF expression through the yeast genome (Burns et al. 1994; Ross-Macdonald et al. 1999).
The Daily News's EA Baughan felt that Robeson's "dignity is more the effect of his massive frame than the expression of a big and noble soul", while Agate, who thought every Othello had to possess a "fine physique", wrote: "In the physical aspect Mr Robeson largely failed.
