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Examination of the DNA sequences revealed that a single base pair deletion had changed the frame of expression of the protein, leading to a premature stop codon at amino-acid 141 (Fig. 5B, bottom sequence).
This may be due to the slight differences in the time frame of expression and nuclear localization of CycE between the KLN-205 cells and the SCC.
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The recombinant protein in frame of the expression vector pET28a were expressed and purified successfully.
The fidelity of the entire open reading frame of each expression vector was confirmed by automated DNA sequencing.
To confirm accurate insertion into the frame of the expression vector, sequencing chemistry were performed.
For the range of measured diffusion coefficient and a range of binding radii, AM-PARs can accumulate within the PSD within the time frame of LTP expression [ 13].
These results show that AMPARs can accumulate within the PSD from the pool of extra-synaptic receptors in the spine by diffusion within the time frame of LTP expression.
In cases where mCherry fusion occurs out of frame, the expression of fusion products can be unmasked by in situ hybridization with antisense mCherry probes.
It is conceivable that degradation processes may have scarce influence in transient expression systems, as the time frame of recombinant proteins expression is limited to just 9 days before harvesting.
In case 1, one image among five frames of each expression per subject is used for training and the remaining four are used for testing.
The debate was primarily framed as freedom of expression versus the right to dignity, both principles enshrined in South Africa's Constitution.
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