*ORF, open reading frame; LEE, locus of enterocyte effacement; STEC, Shiga toxin producing Escherichia coli.
This peptide prediction is located within a regional Genscan [46] (Figure 3), suggesting an open reading frame at this locus.
Low levels of Myc were found to drive proliferation and tumour formation, whereas high levels triggered the apoptotic Alternative Reading Frame of Ink4a/CDKN2A locus (ARF /p53 tumour suppressor pathway.
Finally, BLASTp analyses against Arabidopsis thaliana gene models used to derive coding intervals were used to verify delineation of coding intervals and frame for each locus.
In contrast, if the issue is seen from a "systemic" frame, then the locus of responsibility is with society or government.
Any discussion about guideline implementation and associated capacity must be framed according to locus of responsibility.
To specifically disrupt NES function we introduced LVIAMDQANA and AVAAMDQANA (denoted Mia1p-MutNES2 and Mia1p-MutNES4, respectively) mutations to the mia1 open reading frame at its chromosomal locus.
A 3.6-kb PCR fragment amplified from pEnt-Emr/Zeo containing the marker cassette and 50 bp of flanking sequence was recombined into the open reading frame of the NKX2-5 locus 26 amino acids downstream of the native ATG site (Fig. S3B, C).
We fused sequences encoding an epitope tag to the 3′ end of the maf1 open reading frame at the native locus to generate maf1.pk.
As described in Materials and Methods, the rAAV vector was designed to insert the 2690 bp promoter-less EGFP-Luc fusion gene into exon 2 of the c-Myc locus in frame with the major c-Myc translational start codon.
