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Only after analysing peptides in terms of gene proximity and frame localisation, was the correct annotation of 13 ORFs and the re-annotation of 44 distinct full-length proteins achieved.
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Wrong localisation framed the cognitive process and biased further information gathering, directly impacting on diagnostic closure thresholds.
Figure 1 Localisation of haemorrhagic event.
5' mapping of the LmjF28.1470 mRNA-derived product amplified by PCR suggests that the protein can be produced, however a shorter version, potentially produced from an in-frame ATG, has a different cellular localisation.
Midline localisation, periumbilical or poorly localised 2.
Figure 12 Scenario iii: seizure with varying localisation.
Assignment of the gene coding for this enzyme has been achieved by expression of a putative open reading frame with sequence homology to another previously reported lysophosphatidylcholine transferase and subsequent analysis of its tissue and subcellular localisation, substrate specificity and identity of the product [49].
To experimentally investigate the subcellular localisation of the PpRpoT3-encoded polypeptide, the putative N-terminal transit peptide was fused in-frame to the GFP coding region.
CI2 was inserted into ncmΔNGFR upstream of the IRES element to generate ncmΔNGFR-CI2 and ncmΔNGFR-nuclear localisation signal (NLS -CI2; the latter coNLS -CI2he NLS of SV40 thentigen platterN-terminally in frame with contains
4. Localisation.
Less globalisation and more localisation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com