How bad could a little glory be? "Nobody paid attention to me, it was all about Hannah," said Master Sgt. Joseph Myers, of the video in June that vaulted onto national broadcasts showing the reaction of his 10-year-old — freeze-frame expressions ranging from incredulity to ecstatic relief — when he walked into her Randolph Elementary School class at Universal City, Tex.
When protein traps happen with reporter fusions in frame, mCherry expression exhibits endogenous protein expression information to some extent.
TRMT2A was chosen for study based upon its identification as a novel open reading frame whose expression varies during the cell cycle similar to the oscillation of MCM, tubulin, CDC2, and other genes whose expression peak in S-phase and decrease during mitosis.
If the defender does not reply within a time frame, an expression of derision is witnessed.
Examination of the DNA sequences revealed that a single base pair deletion had changed the frame of expression of the protein, leading to a premature stop codon at amino-acid 141 (Fig. 5B, bottom sequence).
The Pi7C gene is probably functional; all four Pi7C alleles have a full length open reading frame, active expression, and greater conservation within the domain regions than within the inter-domain regions.
In cases where mCherry fusion occurs out of frame, the expression of fusion products can be unmasked by in situ hybridization with antisense mCherry probes.
cDNA sequences of the salivary proteins SP01B and SP04 were successfully cloned in frame into expression vectors as confirmed by sequencing.
This may be due to the slight differences in the time frame of expression and nuclear localization of CycE between the KLN-205 cells and the SCC.
Gene fusions can occur through both intra- and inter-chromosomal translocations, bringing together coding regions from two or more genes within a single reading frame allowing expression of a novel protein.
