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In frame deletions of sigT (CC_3475), sigU (CC_2883), phyR (CC_3477), HK4 (CC_3474), CC_3466 and CC_1532 were obtained by a two-step sacB counterselection procedure, as previously described [64].
We have characterized 11 dplv mutations and most either directly generate a stop codon or are out of frame deletions (see Table 3).
However, since the mutants were generated by precise, in frame deletions that were verified by sequencing, we consider very unlikely the possibility of polar effects.
Neither of the programs can be applied to in frame deletions.
The two in frame deletions were not found in 100 control chromosomes tested.
A small number of mutations involve in frame deletions or insertions.
Similar(52)
We identified splice alterations in intronic regions of APC and large in-frame deletions in CTNNB1, increasing oncogenic WNT pathway alterations to 96% of CRCs.
Duchenne muscular dystrophy is characterized by an absence of functional protein, whereas Becker muscular dystrophy, commonly caused by in-frame deletions, shows synthesis of partially functional protein.
To create unmarked, in-frame deletions, the kan cassette was removed using FLP recombinase [83].
All 20 mutants described henceforth were generated as in-frame deletions of the target genes.
This demonstrates the usefulness of the approach for markerless in-frame deletions.
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