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CycTools implements frame deletion using the Pathway Tools API method "delete-frame-and-dependents".
In frame deletion mutants were constructed by overlapping PCR as described previously [61].
Dpolv12B1 is a very large in frame deletion which removes a large portion of exon 76.
This was an out of frame deletion that also overlapped the translational start site for motB.
After cloning the in frame deletion fragment in pJRS233, the plasmid pJRS233Δsap was obtained.
Such an in frame deletion should not influence the expression of the riboswitch LysRS and of the lysine transporter Lmo0798.
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The fragment deleted for each in-frame deletion is indicated.
In this study, we obtained a bagI deletion mutant by in-frame deletion.
a Schematic representation of the in-frame deletion of ctcH and ctcJ.
Other mutations include nonsense mutations, missense mutations and in-frame deletion (Everett, 2011).
Moreover, mutations from in-frame deletion of TRPML1 greatly influence the channel integrality.
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