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To further study its role in Xcc, the coding region (33 to 280 aa) of sir2x was in frame deleted in the chromosome of Xcc strain XC1 and the resultant mutant was named as Δsir2x.
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A vector for targeted deletion of celA, pJFW52 (Additional file 1: Figure S1), was constructed by joining 1 kb of the upstream and 1 kb of the downstream region of the CelA (Cbes1867) open reading frame, deleting the open reading frame itself.
The newly detected band of 148 bp (variant-4; Var-4) corresponds to a mRNA that is in-frame deleted of exons 5 plus 6 and expected to result in a 139-kDa protein (Fig 1).
The 30 bp in-frame deleted FVII mRNA would encode for a protein lacking 10 residues (Val218-Gln227; chymotrypsinumberingng 21 30) in the activation domain of the serine protease (Fig. 4A).
This introduced multiple stop codons in the reading frame of env after the end of vpu reading frame, and deleted the expression of env.
In that frame you deleted earlier, drag the picture you edited into that frame.
For deletion of putative target genes, the open reading frame was deleted by a PCR stitching method as described in detail in Kwon et al. (2012).
If no argument is given then the current frame is deleted, otherwise the argument determines what to delete.
The HA polybasic cleavage site as well as the NS1 open reading frame were deleted.
The myostatin gene was isolated from mouse cDNA and the C terminal region from nucleotide 825 1131 of the reading frame was deleted via PCR.
In the icl1/icl1 mutants, codons 121 430 of the ICL1 open reading frame were deleted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com