Sentence examples for frame alterations from inspiring English sources

The fact that no stop codons or reading frame alterations were detected in the coding sequences, together with evidence from X. tropicalis EST collections, support the idea that all these genes are transcriptionally active.

In this paper, we report the identification of five novel mutations, three missense variations in exons 1, 16, and 30, and two in frame alterations of exons 20 and 24, thus extending the spectrum of mutations associated with MYH9-RD.

Regarding the two in frame mutations, it is interesting to note that other in frame alterations, two deletions (c.3164_3205del/p.Gly1055_Gln1068del and c.3195_3215del/p.Glu1066_Ala1072del) and one duplication (c.3195_3215dup/p.Glu1066_Ala1072dup) have previously been identified in exon 24 [14,15].

Conversely, in the EZH2-dependent cell lines, almost all clones had at least one fragment length that was 394 bp, indicating the presence of un-edited or in-frame alterations (Fig.  3b, c and d and Additional file 1: Figure S4).

After CRISPR Cas9 targeting, our assay is designed to examine 1) whether the cells that continue to proliferate in a target-dependent cell line are those that retain at least one in-frame allele (either edited or unedited) and 2) whether cells that continue to proliferate in target-independent cell line have alleles that are mostly edited and have out-of-frame alterations.

In order to test our hypothesis that the cells that grow out after 20 days are those in which EZH2 has either not been targeted, or in which the indels lead to in-frame alterations, we developed a medium throughput assay to analyze 10s 100s of clones for gene essentiality.

Regardless of the specific COMP genomic DNA mutation, all the deletions, insertions and indels cause in-frame alterations to the COMP protein primary sequence and no frame shifts have been identified with one exception, which we did not include in this study as our focus was on qualitative protein defects.

We have clarified that the juxtramembrane splicing event giving rise to JM-a and JM-b results from an in-frame alteration in which 75-nt encoding 24 amino acids is replaced with an unrelated 45-nt sequence encoding 14 amino acids.

The physical location of the JM-b insertion is shown in Figure 2. The ErbB4/JM-b isoform was originally defined by an in-frame alteration in which 69-nt in the extracellular juxtamembrane domain of the ErbB4 gene was replaced with an unrelated sequence of 39-nt (9).

Li et al. (2014) suggested that there was a pseudogenization event for PIN in both penguins and reported several stop codons and frame shift alterations [ 25].

The present data and previous studies demonstrated that all oxidants examined resulted in apoptosis in RLE cells during the time frame where alterations of mitochondrial gene expression were observed.