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Further, the fragrant accession 'Velchi' has additional 5 bp duplication as compared to non-fragrant accessions.
This screening experiment revealed that non-basmati fragrant accession 'Velchi' does not harbour any reported mutation in coding region of badh2.
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Multiple mutations identical to fgr allele identified by Bradbury et al. [ 22] were also observed in all the 13 fragrant accessions (data not shown).
In present experiments, we sequenced the OsBADH2 locus derived from a number of aromatic rice cultivars across China and uncovered that multiple mutations identical to fgr allele also occurred in all the tested fragrant accessions.
The results showed that multiple mutations identical to fgr allele occur in the 13 fragrant rice accessions across China; OsBADH2 is expressed constitutively, with less expression abundance in mature roots; the disrupted OsBADH2 by RNA interference leads to significantly increased 2-acetyl-1-pyrroline production.
The new allele has 253 bp deletion in the promoter region of fragrant indica rice accessions compared to non-fragrant rice.
In the present study, selected indica rice accessions (basmati, non-basmati fragrant, and non-fragrant) were screened for mutations in betaine aldehyde dehydrogenase 2 (badh2) gene to investigate the allelic differences using previously reported functional markers.
We examined genetic variation in a 463 bp portion of OsBADH2 gene in 13 fragrant and six non-fragrant rice accessions that are representatives of cultivated varieties across China (Table 1).
To distinguish Velchi from other non-fragrant indica accessions, a new marker (velbadh2-p-UTR) has been designed to identify this 5 bp difference, which could distinguish 'Velchi' from non-fragrant rice accessions and can be useful in breeding for development of fragrant rice varieties via marker-assisted selection.
Fragrant moss.
"It's lightly fragrant.
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