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In this work, two model DNA nanostructures (i.e., Nan and Kai) were designed based on the shape of the two Chinese characters of the name of Nankai University, and additional single-stranded DNA fragments were added to interact with graphene.
Hence two dif fragments were added to both sides of the resistance gene, which were recognized and cut-out by Xer recombinases, obtaining the label-free mutant strains (Cascioferro et al. 2010).
Following gel purification of PCR products, these were combined and used in amplification reactions in the absence of primer for 5 cycles, at which point primers targeting the ends of the fragments were added and amplifications continued for 30 cycles.
The Blast scores for disrupted gene fragments were added to give an overall score.
The dangling ends of synthesized fragments were added with XbaI restriction sites (Takara, China).
GFP cDNA fragments were added to the probes in equal amounts, before labeling, to use as positive controls.
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Populations of DNA fragments are added to slides and placed inside the device.
Random fragments are added and removed from reactants and products such that in the end a different reaction is obtained that has the same reactive center.
A dilution series of the labeled Ab and fragments was added to 0.5 × 106 cells and incubated for 2 h at 4°C.
The predicted structures of the metabolites can be used for the assessment of pharmacological and toxicological profiles and in mass spectrometry for the assessment of the positions where chemical fragments are added to or removed from the substrate structures.
We have therefore developed SASI-Seq; a process whereby a set of uniquely barcoded DNA fragments are added to samples destined for sequencing.
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