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A prerequisite for the usefulness of the technique portrayed in the present work is that the erratic tumor fragmentation results in fragments that differ widely in their relative content of hypoxia, as quantified by relative FAZA retention.
So-called exotic terranes, geologic fragments that differ markedly in stratigraphy, paleomagnetism, and paleontology from adjoining continental crust, were accreted to the margin of the North American plate.
Specific primer pairs were used for amplifying the corresponding ancestral fragments that differ from the extant plastomes in genomic organization (fig. 3).
Initially, suppression subtractive hybridization has been applied on a cohort of libraries of PCR-amplified cDNA fragments that differ between control (normal) and experimental (cancer) transcriptome [ 28].
Typing can be performed by amplifying this region with PCR using primers flanking this region and running the PCR product on a high-resolution acrylamide gel that can separate fragments that differ in size by a few base pairs (33 ).
For VDAC, we have observed ten markers in the case of the animal sequences and some of them are present within four characteristic fragments that differ in length (underlined sequences in table 2).
Similar(53)
The successful PCR cases are divided into three categories: similar (DNA fragments predicted correctly), different (DNA fragments that differed significantly from prediction), and false (no polymorphism).
On the other hand, amplified fragments that differed in presence/absence EcoRI/ HpaII and EcoRI/ MspI patterns in at least one genotype were considered as "methylation-sensitive polymorphisms".
We visually checked the automatically created bins to ensure the bin was centered on the distribution of peaks within the bin and removed bins that had AFLP fragments that differed in size by more than 1 bp.
As it is well known that the DNA extraction method is critical for the quality of a DNA sample, we tested two different extraction procedures and finally evaluated the three preservation methods based on the success in PCR amplifications targeting four DNA fragments that differed in length.
By examining genetic relationships among E. coli isolates from humans, livestock, and 3 species of primates, we inferred rates of bacterial transmission among populations of these species living in or near 3 fragments that differed in their degrees of anthropogenic disturbance.
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