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Labeled aRNA was purified and fragmented using fragmentation reagents from Ambion.
The circularized fragments were fragmented using Covaris sonicator.
mRNA was enriched using beads with oligo (dT) and fragmented using fragmentation buffer.
Labeled aRNA was then fragmented using Ambion Fragmentation Reagents (Ambion).
The next day, the DNA was enzymatically fragmented using end-point fragmentation to avoid over-fragmentation.
Briefly, ribosomal RNA-depleted sample was fragmented using an RNA fragmentation solution prior to cDNA synthesis.
The mRNA was randomly fragmented using the RNA fragmentation kit from Ambion.
The mRNA was chemically fragmented using the Ambion RNA fragmentation kit (Life Technologies).
Briefly, DNA was fragmented using sonication and the resultant fragment distribution assessed on an Agilent BioAnalyzer 2100 platform.
Here, compounds are fragmented using tandem MS, for example by Collision Induced Dissociation (CID).
DNA molecules are amplified and then fragmented using techniques such as sonification or nebulisation.
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