Sentence examples for fragmented use of from inspiring English sources

Given the lack of an educational substructure for EBP in the curriculum, the informants separately described similar themes of arbitrary integration of EBP content across the different preprofessional intervention and research courses, and fragmented use of teaching-learning and evaluation methods.

The pooled PCR products were fragmented using 0.05 U of fragmentation reagent (Affymetrix) at 37°C/10 min, followed by inactivation at 95°C/15 min. The fragment size was analyzed using chip electrophoresis (Bioanalyzer DNA 1000 Nano Assay; Agilent), and the average size was 50bp (range 20 200bp).

Total RNA (5 μg) from both cell lines were depleted of 18S and 28S rRNA and 1 μg of each sample were enzymatically fragmented using 1 unit of RNase III (Ambion) and incubating at 37°C for 10 minutes.

Sample (in 30 μL of elution buffer) plus 0.5 μL of bovine serum albumin (New England Biolabs), 5 μL of 10× Buffer 4 (New England Biolabs) and 28 μL of DNAse/RNAse free water were divided into two equal fractions and fragmented using 10 U of MseI (New England Biolabs) as follows, 16 h at 37°C followed by 65°C for 20 min.

PCR products were then purified and fragmented using 0.24 units of DNase I at 37°C for 30 minutes.

For ESI-MS/MS, selected ions were fragmented using collision energy of 15 V (phenolic acids derivatives) or 25 V (flavonoids derivatives) and collision gas (argon) at 0.1 mL/min.

aRNA was fragmented using 5x fragmentation buffer and the total amount of fragmented biotinylated aRNA used per chip was 6.5 μg.

To ensure the best fragmentation spectrum for any phosphopeptide was obtained, the precursors were fragmented using multistage activation (MSA) of the precursor mass (m/z) minus 49, 32.33 and 24.25 Da (this represents the loss of H3PO4 from a 2+, 3+ or 4+ precursor ion).

A total of 1 μg of DNA was fragmented using a Covaris S220 sonicator (Covaris Inc). to 250 bp fragments by adjusting the treatment time to 85 sec.

For each experiment, 90 μg of DNA was fragmented using 4 units (8 units for the WGA DNA) of DNase I (New England Biolabs, Ipswich, MA, USA) in a total volume of 500 μl 1× DNase I Reaction Buffer (New England Biolabs).

200 ng of RNA was fragmented using divalent cations (ZnCl2) at 70°C for 30 s.