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For use as unlabelled blocking, genomic DNA of each pollen parent was fragmented up to 100 300 bp in size by boiling in 0·4 m NaOH for 40 min according to the previously described protocol (Cao et al., 2000).
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These materials are poorly sorted and sub-angular to angular rock fragments up to the boulder size of gneiss, quartzite and limestone.
Plagioclase fragments up to 0.9 mm across and large angular fragments of orange-yellow olivine crystals with a maximum size of 0.4 × 0.2 mm are present in the crystalline matrix.
After tailing reaction and amplification of double stranded cDNA by long-distance PCR, fragments up to 500 bp were discarded by chromatography-based size selection.
Importantly, amplification using ExCyto PCR is robust (Figures 2 & 6) and yields PCR fragments up to 3,000 bp from single colonies, bacterial cultures, glycerol stocks, and low copy plasmids.
Then, it uniformly samples paired-end fragments up to a certain abundance level for each selected transcript.
The corresponding cationic ESI mass spectra show fragments up to [(P3C2Mes2)4Cu11Cl6]+, [(P3C2Mes2)4Cu10Br5]+, [(P3C2Mes2)4Cu10I5]+ and [(P3C2 t Bu2)7Cu16Cl8]+, respectively.
Roche 454 implements pyrosequencing, which measures released pyrophosphates allowing the analysis of read fragments up to a few hundred base pairs.
Restriction fragments up to 3 kb were A-tailed using a Taq DNA polymerase (Fermentas), cloned into pGEM-T Vector (Promega), and sequenced with universal vector primers.
We select linear fragments (up to a size of 7 atoms) as features in the wizard.
By the first vulcanian event, hot pumice fragments, up to 15 cm across, and smaller lithic fragments fell about 2.9 km southeast of the crater (Fig. 3(C)).
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