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Pooled DNA was fragmented, to produce DNA fragments of uniform small size (ca. 20 ~200 bp).
The purified lipase-positive fosmids were fragmented to 600 900-bp fragments by nebulization, undertaken according to Roche recommendations.
Following purification, mRNA was fragmented to ~200 base pair fragments and cDNA generated with ligated adaptors.
First, mRNA was isolated with oligo dT) beads and then fragmented to 100 400 bp by fragmentation buffer.
Purified cRNA was fragmented to 200 300 mers using a fragmentation buffer.
mRNA samples were chemically fragmented to 200 250 bp using 1× fragmentation solution for 5 min at 70 °C (Ambion).
Purified cRNA was fragmented to 30 200 nucleotide lengths using a fragmentation buffer.
Amplified mRNA from each sample was fragmented to 100 200 nt using 10× RNA fragmentation buffer (Ambion).
Eluting peptides were detected, isolated, and fragmented to produce a tandem mass spectrum of specific fragment ions for each peptide.
Because the canines in the material were not fragmented to such a degree, we did not consider fragmentations a problem.
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Before library preparation, cDNA samples were Covaris-fragmented to 300-bp fragments.
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