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Like Kawakubo, and, indeed, most of the participants in "Skin + Bones," he dismantled, ruptured, fractured, or fragmented, then reconfigured, not only clothing or buildings but the traditional logic behind them, which suddenly ceased to seem inevitable.
Briefly, mRNA was extracted using oligo dT beads, fragmented, then reverse transcribed using random hexamers.
RiboZero depleted RNA was chemically fragmented, then made into cDNA with Superscript III (Life Technologies) and random hexamers followed by a second strand reaction.
If the set of viable metabolisms is disconnected and highly fragmented, then historical contingency becomes important and restricts the alteration of metabolic properties, as well as the number of novel metabolic phenotypes accessible in evolution.
Dye-labeled RNA was fragmented, then diluted in a 50 µl solution containing 3× SSC, 25 mM HEPES-NaOH, pH 7.0, 20 µg human Cot-1 DNA (Invitrogen), 20 µg poly(A) RNA (Sigma-Aldrich, St.Louis, MO), 25 μg yeast tRNA (Invitrogen), and 0.3% SDS.
The photos get cut up, deconstructed, fragmented then reformed merging the various buildings together to form multi-layered hybrid shapes.
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Flocks of billions that took days to pass dwindled to fragmented populations, then a smattering of individuals, then a few in captivity.
Briefly, embryos were mechanically fragmented and then incubated with trypsin (0.25% in PBS pH 7.5) at 37°C for 15 20 minutes with a magnetic stirrer.
Fragmented DNA then underwent end repair using New England Biolabs (Ipswich, MA) NEBNext End Repair Module following manufacturer's instructions.
In brief, polyA mRNA was isolated from total RNA, chemically fragmented, and then reverse transcribed to form double-stranded cDNA.
The advantage of MS/MS is that peptides are fragmented and then identified by specific amino acid sequences.
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