Sentence examples for fragmented template from inspiring English sources

Amplification products from FFPE DNA resulted in fragmented template <500 bp in length (data not shown).

APEX array technology facilitates the simultaneous analysis of multiple mutations through hybridization of fragmented template DNA to specifically designed primers, followed by single nucleotide extension at the site of each putative mutation [8], [9].

Briefly, fragmented genomic template DNA was denatured, mixed with Thermo Sequenase DNA Polymerase USB Corporationn) and fluorescently labeled dideoxynucleotides before being applied to microarray slides for a 20 minute isothermic APEX reaction.

The fragmented, genomic template for this step is limited so that amplification is digital for most reactions; roughly 40,000 reaction droplets were inoculated per patient to ensure that the final PCR products were consistent and normalized.

Whenever circulating DNA is analyzed, copy numbers derived from a real-time PCR assay with fragmented DNA as a template cannot easily be interpreted as haploid whole genome equivalents because the real-time PCR assay counts only the copies of available full length target.

This was offered by the advent of 'next-generation sequencing (NGS)', also termed 'deep sequencing', whereby the entire genomic template is fragmented, sequenced in short reads and then reassembled through complex bioinformatic comparison with a genomic database (Ref. 50).

MicroPoly(A Purist kit (Ambion) was used to isolate mRNA from each total RNA pool and 200 ng of mRNA were fragmented and used as template for double stranded cDNA production using cDNA Synthesis System Kit (Roche) followed by adaptor ligation.

The present study describes a simple and inexpensive method for generation of semi-randomly fragmented DNA from amplicon templates.

Organisational barriers included professionals having difficulty accessing PAAP templates and fragmented processes including patients not being asked to bring PAAPs to their asthma appointments.

This is echoed in work around primary care for LTCs where reductionist approaches driven by templates and fragmented division of labour marginalises the ability of practitioners to develop self-management dialogues with patients [ 29].

Biotin-labelled cRNA was subsequently synthesized on cDNA templates and fragmented prior to hybridization to Affymetrix GeneChip HG-U133 plus 2 slides following the manufacturer's recommendations (Affymetrix, Santa Clara, CA, USA).