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The quality of the total RNA, cDNA and fragmented targets was determined using the Agilent 2100 Bioanalyser according to manufacturer's instructions.
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Twenty μg of purified cRNA was incubated in Affymetrix fragmentation buffer at 94°C for 35 minutes and 150 ng of fragmented target was assessed using the RNA 6000 Nano Kit, Agilent 2100 Bioanalyzer.
Participants were presented with a modified block design task (BDT), in both a typical visuo-constructive version that involves reconstructing figures from blocks, and a perceptual version in which respondents must rapidly match unfragmented figures with a corresponding fragmented target figure.
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Their technology has been adopted by marketers looking to shift their marketing strategy, moving away from audience segmentation and fragmented targeting to build more complete profiles of their consumers.
The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA), and herring sperm DNA [17].
This typically resulted in fragmented target with a size range between 100 200 bases.
At higher E/T ratio (20/1), the 51Cr release from the target cells increased to a greater extent than the percentage fragmented target cell DNA.
Biotin-labelled and fragmented target cRNA samples were loaded into Affymetrix GeneChip (Human Genome U219) Array Strips together with controls cRNAs and oligo B2.
Approximately 160 ng of the fragmented target (estimated from concentration of starting material) was assessed using the Agilent 2100 Bioanalyzer to determine population size relative to known markers.
4 μg of the fragmented target was hybridized at 45°C with rotation for 16 hours at 60 rpm to probe sets present on an Affymetrix E. coli 2.0 array.
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