A limitation of Yeast 1.0 came about through the very generation of the consensus; the network became considerably fragmented as reactions that could not be readily annotated (due to the presence of structural ambiguities) were removed.
Pushing factors include risk reduction, response to diminishing factor returns in any given use, such as family labour supply in the presence of land constraints goaded by population pressure and fragmented land holdings, reaction to a crisis or liquidity constraints, high transaction costs that influence households to self-provision in several goods and services.
We live in an age of fragmentation: fragmented communities, fragmented lives, fragmented attention.
90 μg of PCR product was fragmented with Fragmentation Reagent (Affymetrix) at a final reaction concentration of 0.005 U/μL.
At the back of MacGregor's lecture I also waited for him to acknowledge the statist and centralising forces which grew in brutal reaction to the fragmented chaos of medieval Germany.
The precise amount of DNase added per sample depends on the batch of enzyme: our goal was to calibrate the reaction to obtain fragmented DNA of approximately 50 bp, with low variance in fragment size.
cRNA was fragmented prior to the hybridization reaction as previously described [ 10, 26].
The reaction mix containing fragmented DNA and PCR mastermix was dropletized and merged with the droplets containing the primers through a high throughput microfluidic emulsion PCR system using a RainDance Thunderstorm.
Charnley [ 24], in his studies of the natural history of patients after total hip replacement, was the first to describe the presence of a 'macrophage foreign body reaction' associated with fragmented methylmethacrylate cement in peri-implant tissues from loosened prostheses.
To assess recovery of the exogenous spike-in from cfDNA extractions, a four-point tenfold dilution series (from 50,000 to 50 copies per reaction) of the fragmented ADH plasmid (diluent, nuclease-free water) was used to measure the number of copies of each ADH plasmid fragment.
In the reaction, molecular oxygen is fragmented into two oxygen atom radicals, which react with other oxygen molecules to form ozone.
