For the detection of apoptosis by fragmented nuclei observation, MEFs were cultured on coverglasses coated with poly- L-lysine (Sigma).
Though chronological, its form is fragmented: snatches of conversations, observations and impressions.
Therefore, caution is needed in the interpretation of the results, considering the fragmented nature of the observations (see also [3]).
The observations of fragmented knotworking [ 10] and suboptimal interprofessional collaboration [ 14] in earlier research may be apparent because there was not a role, such as the NP, integrated into these teams.
WT cells have several small dots, crescent-shaped, or spherical YFP-LC3 puncta representing isolation membranes and autophagosomes, respectively, that were observed on or near fragmented mitochondria, consistent with previous observations.
These findings together with the observation of fragmented mitochondrial morphology in pulmonary smooth muscle cells of PAH patients raised some speculation on a pathogenetic contribution of mitophagy in PAH [58].
At present, it is unclear, whether our observation of fragmented and small NMJs in dilute mice could partially explain their severe locomotor defects, in particular because previously also a correlation between aetiopathology and functional defects in the cerebellum of myosin Va deficient mice and rats was described [27], [45], [46], [but see for conflicting results 47], [48].
The financial services industry, by my observation, has become fragmented since internet competitors entered the scene.
We expect P-clouds to be more sensitive than homology-based methods for identifying more divergent and fragmented repeat elements, and the observation that the estimated python repetitive content is nearly twice as high based on P-clouds analysis indicates that much of its repetitive content may be older and/or more fragmented than that of the copperhead.
These observations suggest that these fragmented IFP will be useful for reporting on protein protein interactions within animal models, which are hard to study using fragmented GFP.
