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In small isolated populations, extrinsic natural and anthropogenic factors (e.g. habitat loss and fragmentation, use of pesticides) may lead to a decrease in population size, resulting in frequent inbreeding, increased diploid male production and ultimately population extinction [28].
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Results are presented as the percent of specific DNA fragmentation using the formula: (percentage of experimental apoptosis−percentage of spontaneous apoptosis)/(100−percentage of spontaneous apoptosis) × 100.
Apoptosis was detected by DNA fragmentation using TdT incorporation of nucleotides on 3′ ends of DNA (TUNEL technique).
After 18 cycles of second strand synthesis and amplification, cDNA was purified with a 1× Agencourt AMPure XP bead cleanup (Beckman Coulter), and the Nextera XT DNA Library Preparation kit (Illumina) was used for sample fragmentation/barcoding. Fragmentation used 1 ng of purified cDNA, followed by 12 cycles of amplification prior to unique dual-index barcoding.
Based on previous works about production fragmentation and use of imported inputs, it is expected that the purchase of parts and components from another country should provide some efficiency gain or advantage to Latin American countries.
Results are presented as % specific DNA fragmentation using the formula: (percentage of experimental apoptosis−percentage of spontaneous apoptosis)/(100−percentage of spontaneous apoptosis) × 100.
In this study we interrogate multiple sequence signals that appear due to the non-random nature of cell-free DNA fragmentation using high coverage sequencing of maternal plasma.
Mass spectra were acquired over m/z 300 1750 at 70 000 resolution (m/z 200) and data-dependent acquisition selected the top 12 most abundant precursor ions for MS/MS by HCD fragmentation using an isolation width of 1.2 m/z, normalized collision energy of 30% and a resolution of 35 000.
Second, while 98% of all compounds in the NIST11 MS/MS library were reported using more than one fragmentation energy, the use of fragmentation energies was not systematically performed.
The top 3 most abundant ions were selected for CID fragmentation using an isolation width of 1.5 m/z, a normalized collision energy of 35, an activation time of 30 ms, and an activation Q of 0.250.
The second case involved measurement of fragmentation using Split Desktop in Goltas limestone quarry.
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