Sentence examples for fragmentation times from inspiring English sources

Fragmentation times were adjusted to 15 sec.

Fragmentation times were adjusted to (a) 60 sec and (b) 15 sec to create libraries with Mate-Paired reads 300-900 bp apart (with a maximum at 800 bp).

The shorter fragmentation time was used to yield slightly larger library fragments of 350-400 bp.

First, we shortened RNA fragmentation time to increase overall insert lengths.

The fragmentation time of 5 min is the standard time used in the protocol, which yields fragments of ~250 bp.

The fragmentation parameters were as follows: duty cycle, 10%; intensity, 5; cycles/burst, 200; cycles, 16; and total fragmentation time, 960 sec.

Strand-specific cDNA libraries were prepared using the Bioo Scientific NEXTflex Directional RNA-Seq Kit (dUTP Based) for Illumina following manufacturer instructions with a 12-min fragmentation time and 15 PCR cycles.

To take advantage of increased read length of >150 nt, we demonstrated shortened RNA fragmentation time, which resulted in a dramatic shift of insert size distribution.

Several covariates that did not come out significantly were not included in the final model (alcohol use, education level, time between RNA amplification and RNA fragmentation, time between RNA fragmentation and RNA hybridization).

After confirming the reproducibility in a different species (data not shown), we shortened RNA fragmentation time from eight to two (Library B, C, and E) or four (Library F H) minutes.

Samples were prepared for multiplex sequencing using Illumina TruSeq RNA Sample Prep Kits A and B (part # FC1221002 (kit B), Lot: 5781467) according to manufacturer's instructions, with an RNA fragmentation time of 8 minutes at 94°C.