"Fragmentation time" is not technically correct in the context of written English, as it is an informal phrase.
However, it is very commonly used in informal speech. For example, you might hear someone say, "It's been a long day - I think it's fragmentation time." This means that it is time for a break, such as a short nap.
The shorter fragmentation time was used to yield slightly larger library fragments of 350-400 bp.
First, we shortened RNA fragmentation time to increase overall insert lengths.
The fragmentation time of 5 min is the standard time used in the protocol, which yields fragments of ~250 bp.
The fragmentation parameters were as follows: duty cycle, 10%; intensity, 5; cycles/burst, 200; cycles, 16; and total fragmentation time, 960 sec.
To take advantage of increased read length of >150 nt, we demonstrated shortened RNA fragmentation time, which resulted in a dramatic shift of insert size distribution.
Strand-specific cDNA libraries were prepared using the Bioo Scientific NEXTflex Directional RNA-Seq Kit (dUTP Based) for Illumina following manufacturer instructions with a 12-min fragmentation time and 15 PCR cycles.
Fragmentation times were adjusted to 15 sec.
Fragmentation times were adjusted to (a) 60 sec and (b) 15 sec to create libraries with Mate-Paired reads 300-900 bp apart (with a maximum at 800 bp).
The fragmentation (FRAG) time point consists of 4 samples that contained a similar proportion of meiotic cells 20-299%) as the MEI time point, but also contained 37-43% of cells with a fragmented old MAC.
Using archived aerial imagery from 1958, 1995 and 2007, for two consecutive periods (1958 1995 and 1995 2007), we calculated a set of landscape metrics to compare woodland fragmentation over time.
Genetic differentiation among populations was greater in the smaller tree group than in larger one, suggesting that more recently established individuals are exposed to greater genetic isolation than the mature individuals owing to increasing forest fragmentation over time.
