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In addition to its use for library construction and sequencing, new ultra-frequent DNA fragmentation technology based on the unique "affinity star activity" (i.e., relaxed sequence recognition) of some of the Thermus sp. family enzymes may be useful for other cloning applications.
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But different samples generated from different proteases or analyzed with different fragmentation technologies could be used to train the Riptide models.
However, these effects vary depending on the fragmentation of technology fields and whether the firm operates in a discrete or complex product industry.
A novel competitive binding assay based on enzyme fragmentation complementation technology was established to screen the binding affinities of emerging chemicals for estrogen receptor (ER) α or β isoforms.
The alternative is a fragmentation of technologies.
It was spotted onto a Prespotted AnchorChip (PAC) MALDI target plate (Bruker) and analyzed using methods optimized for peptides and for fragmentation using LIFT technology.
Subsequently 1.65 μg Cy-dye-labeled cRNA was fragmented (mean size, approximately 50-100 nucleotides) with fragmentation buffer (Agilent Technologies) at 60°C for 30 min; cRNA was subsequently hybridized to the microarray at 65°C for 17 h.
Then, 0.825 μg of the Cy-labeled cRNA was fragmented to an average size of about 50-100 nucleotides by incubation with the fragmentation buffer (Agilent Technologies) at 60°C for 30 min. Subsequently, the fragmented labeled cRNA was pooled and hybridized to the Whole Rat Genome microarray at 60°C for 17 h.
Then, 0.825 μg of Cy-labeled cRNA was cut into fragments of approximately 50 100 nucleotides by incubation in the fragmentation buffer (Agilent Technologies) at 60°C for 30 min.
Cy-labeled cRNA (2 μg) was fragmented to an average size of about 50 100 nucleotides by incubating with fragmentation buffer (Agilent Technologies) at 60°C for 30 minutes.
One to 5 µg of each labeled target aRNA were mixed with 9 µL of 25× fragmentation buffer (Agilent Technologies) and the final volume adjusted to 225 µL with RNAse free H2O followed by incubation for 30 min at 60°C.
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