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The overall temporal fragmentation pattern of natural forests had a "perforation-subdivision/shrinkage-attrition" pathway, which corresponded to Forman's landscape fragmentation rule, while the plantation forests did not follow the rule strictly.
Constantly, new rules are discovered that have to be added to the fragmentation rule databases.
In particular, the RDA dissociation was the most common fragmentation rule which might correspond to the fused six-membered rings in their structures.
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Rule-based fragmenters are based on fragmentation rules that were extracted from the MS literature over the years.
With regard to filtering, Azure Classic as well as Google have changed their fragmentation rules; and tiny fragments are now filtered at all providers.
When ion 3b (m/z 491) was selected as the precursor ion to perform MS2 experiment, prolific fragment ions were obtained, from which their fragmentation rules were proposed as shown in Fig. 4.
In addition, we implemented fragmentation rules which reflect the (retro- synthetic access to these molecules.
The commercial software Mass Frontier [12] uses rule based fragmentation prediction based on standard reactions, a comprehensive library of over 100,000 fragmentation rules, or both.
Based on the above experiments, the fragmentation rules for swerilactone K (4) in negative mode were concluded.
In contrast, rule-based prediction of fragmentation spectra (see Section "In silico fragmentation spectrum prediction") requires expert-curated "learning" of fragmentation rules.
A third commercial tool is MOLGEN-MS[196, 197] that uses general mass spectral fragmentation rules but can also accept additional fragmentation mechanisms.
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