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Rather than to fragment the original molecule, our fragmentation method first builds an intermediate reduced graph where all the nodes represent a structural unit of the original molecule.
Rather than fragment the original molecule, the fragmentation method first builds an intermediate reduced graph where all the nodes represent a structural unit of the original molecule.
The peptide moiety of a glycopeptide and the peptide without attached glycan should, in theory, produce the same experimental fragment ions because HCD is the fragmentation method applied.
The Covaris fragmentation method has been shown both to produce narrow fragment distributions as well as giving a better recovery of sample [6].
As these results are driven more by the metadata used than the fragmenter behind, a variety of entries were created to assess the contribution of the individual metadata aspects, as well as a "Combined Fragmenter" entry (Combined MS/MS) to remove the influence of the fragmentation method (see "Methods" for details).
A set of 2794 compounds with measured intrinsic aqueous solubility (log S) was fragmented into rings, linkers, and R groups using a controlled hierarchical fragmentation method.
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HCD is one of the fragmentation methods that can generate peptide fragment ions.
Gene fragmentation methods, such as CDH, thus hold great promise for tackling difficult-to-express target proteins.
The major advantage of complementary peptide cleavages by combining different fragmentation methods is the increased amount of information that is obtained during MS/MS analysis of modified peptides.
A gel-based mass spectrometry strategy with ETD and CID fragmentation methods were used to sequence and determine the presence/location of any PTMs on the spidroin-1.
Our results show that the fragmentation methods CAD and ECD allow for a facilitated analysis of phosphopeptides; however, their general applicability for analyzing phosphopeptides has to be evaluated in each specific case with respect to the given analytical task.
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