Exact(4)
After fragmentation, libraries were constructed according to the Illumina Paired-End protocol.
This package allows the user to readily generate large-scale in silico fragmentation libraries and perform mass spectral matching to mass spectrometry-derived data.
Following fragmentation, libraries were constructed using the Illumina Paired-End protocol consisting of end repair, < A > base addition and methylated-adaptor ligation.
In silico fragmentation libraries would improve the identification of compounds from experimental multistage fragmentation data when experimental reference data are unavailable.
Similar(56)
Mass Frontier (see below) currently contains the largest fragmentation library, manually curated from several thousand publications [33].
PCR products were directly sequenced by Ion Torrent PGM sequencer (Life Technologies) after fragmentation, library preparation, purification and cycle sequencing according to manufacturer's instructions.
In summary, the WGA process was divided into fragmentation, library generation, and PCR amplification.
Fragmentation, library construction, and sequencing were carried out by the National Instrumentation Center for Environmental Management NICEMM; Seoul, Korea).
Totally 11 primary metabolites significantly varied between the two extracts were identified based on MS fragmentation, library, and standards.
The large fragmentation library can be easily adjusted and applied to other specific studies, such as lipidomics, environmental analytics, pharmaceutical analysis, and plant metabolomics.
It includes three resources that assist the user to robustly predict in silico fragmentation patterns: a set of general fragmentation rules, a fragmentation library comprising ∼150 000 fragmentation mechanisms collected from the scientific literature and user-specified mechanisms.
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