Sentence examples for fragmentation assays from inspiring English sources
The occurrence of apoptosis-mediated cell death was confirmed using PARP cleavage assays where full length PARP (116 kDa) was cleaved into a smaller 89 kDa fragment through caspase-3 activity, while DNA fragmentation assays indicated a 150 kb to 200 kb laddering of DNA as early as 12 h upon ACA exposure, which is a strong hallmark of apoptotic events.
In addition, mitochondrial membrane potentials and DNA fragmentation assays confirmed that YES-2/AS-12 was more resistant than YES-2/Neo to apoptosis induced by cisplatin.
As CQ-induced parasites displayed clearer PCD induction and inhibition in MOMP, cysteine protease activity and in situ DNA fragmentation assays, we focused on the characterization of the CQ-induced pathway and its mediators.
Both the apoptotic death of chondrocytes caused by anti-Fas antibody and its potentiation by HIF-2 α expression were also confirmed by DNA fragmentation assays (Supplementary Figure 3a).
Occurrence of apoptosis was assessed using PARP and DNA fragmentation assays, while the mode of action were elucidated through global expression profiling followed by Western blotting and IHC assays.
To further analyse the consequence of BLT2 inhibition in paclitaxel-induced apoptosis, we performed sub-G1 analysis, DNA fragmentation assays, and PARP cleavage assays after exposure to LY255283 or BLT2 depletion by RNAi.
DNA fragmentation assayed with the clamped homogeneous electric field (CHEF) technique was used as a measure for dsbs in DNA.
DNA fragmentation assay showed fragmented bands indicating that the mechanism of cytotoxicity is through apoptosis.
Distinct fragmented band observed in DNA fragmentation assay signifies that the mechanism of cytotoxicity on cancer cell is apoptosis.
Chondrocyte apoptosis was also determined by DNA fragmentation assay and flow cytometry based on the number of cells containing fragmented DNA.
Biochemically, apoptosis is characterized by activation of endogenous nucleases and DNA degradation into fragment multiples of 185 bp [ 24] which we observed in the DNA fragmentation assay.
