Suggestions(5)
Exact(8)
With the limited and fragmentary sequence information available for the Oryza species (Kim et al. 2008), we employed a combination of homology-based and de novo methods for repeat detection and categorization.
In some poorly sampled but significant ingroup clades we also included species with fragmentary sequence data.
Conflicts can arise if a TrEMBL AC reflects a fragmentary sequence, which is then (by Ensembl/BioMart) associated with multiple genes and therefore could be assigned to multiple SYSTERS families.
However, the precise phylogenetic location of tunicates inside chordates started to become a matter of controversy in more recent years since new phylogenetic inferences based on morphology or fragmentary sequence data suggested conflicting interpretations (reviewed in Swalla and Smith [2008]).
The exclusion of fragmentary sequence data is especially important in identifying the upstream 5'UTR boundary, as short non-coding RNA sequences are often positioned immediately upstream of the 5'-end of coding genes [ 57].
For example, many protein sequences have arisen by accretion of common protein domains in different combinations (Vogel et al., 2004), and some high-throughput sequencing strategies such as metagenomic shotgun sampling generate fragmentary sequence data (Schloss and Handelsman, 2005).
Similar(52)
The MakeTable5 program also eliminates redundancies, closely similar sequences and fragmentary sequences.
The clustering utilized similarity over large portions of sequence length, rather than just domains, and length-based thresholds were incorporated to address fragmentary sequences and to minimize grouping of unrelated proteins.
Fragmentary sequences were then deleted.
We did not assess the accuracy of phylogenetic placement of the fragmentary sequences.
The majority of the missing true positives correspond to fragmentary sequences in UniProtKB.
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