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20 fractions were obtained after fractionation.

Lysates for nuclear and cytoplasmatic fractions were obtained using a fractionation kit (Biovision) following the manufacturer's instructions.

Cytosolic and nuclear fractions were obtained with the BioVision Nuclear/Cytosol Fractionation Kit.

The gradient fractions were obtained and analysed using a Teledyne Isco fractionation system.

Using a subcellular fractionation and sucrose step gradient, total cell lysates, nuclear and mitochondrial fractions were obtained.

In solubility fractionation studies with sorghum and pearl, finger and foxtail millets, five protein fractions were obtained (Table 23).

By multi-stage separation, standard-grade fractions were obtained.

The absorbance of the eluent was measured at 214 nm, and 20 fractions were obtained, desalted and vacuum-dried for further MS analysis.

Twelve fractions were obtained as follows: F1.1 and F 1.2.

Several fractions were obtained and most active fractions against the R. solani.

After anion-exchange, four protein fractions were obtained, peaks 1 4.