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Bar charts: Comparison of the RNA distribution in non-polysomal (light grey) and polysomal (dark grey) fractions after performing sucrose gradient fractionation without prior cushion purification.
Moreover, offline peptide fractionation in IgYHSA sample allowed a 4-fold increase of proteins identified (520 vs. 131 without fractionation), without hindering reproducibility.
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To further determine whether maspin regulates the cytochrome c release from the mitochondria, TM40D-cont and TM40D-Mp cells were serum starved and the cells were harvested for both whole cell extracts and cellular fractionations without mitochondria.
In this case a cumulative radiation dose is, like for 3D conformal radiation, applied to a given target volume with dose fractionation but without supply of stereotactic coordinates.
It must be remarked that without a method to enumerate purely fractionated kinematic chains it is almost impossible to compare enumeration results with or without fractionation.
By sending the entire sample through without fractionation, the concentrations of all of the lipids remain constant relative to each other.
However, this method depends on corresponding dietary information and also is constrained to the use of one isotope without fractionation.
In our laboratory we developed this technology with the objective to perform a dry fractionation lignocellulose biorefinery without effluent generation.
Some re-oxygenation after light delivery would, of course, be essential for reperfusion injury to play a part in any PDT, with or without fractionation.
A second cell pellet obtained at the same time was homogenized in ice-cold cell disruption buffer without fractionation to give a whole cell lysate.
Throughout this study, a bulk whole blood culture system was employed in which peripheral blood was diluted at a 1 : 1 ratio with culture medium and used without fractionation.
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