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Therefore, application of additional fractionation strategy can improve the proteome coverage.
The subcellular fractionation strategy used to prove FMRP nuclear localization should be improved considering the nature of the protein.
Due to the nature of FMRP, such a fractionation strategy may not be suitable for researching the intracellular localization of the protein.
In this study, we developed an efficient two-step fractionation strategy for the enrichment of iISGs and mISGs, in which an initial discontinuous OptiPrep gradient was applied, followed by a Percoll solution.
Furthermore, the recognition that NOM is composed by supramolecular associations rather than macropolymers has allowed the development of a fractionation strategy, called humeomics, that enables the analytical detection of most of the single molecules which constitute the supramolecular assembly [16-18] [16-18]
Given the size and the extent of the repeat content of the R. microplus genome, the Cot fractionation strategy was chosen to facilitate the generation of sequence information from the gene-containing regions of the genome.
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Currently, different fractionation strategies are applied including mostly chromatography (ion exchange, reverse phase etc).
Multidimensional peptide fractionation strategies have been approved as the efficient approaches to significantly improve the depth of proteome coverage.
Fractionation strategies based on different solubilities of the cork suberin components in chloroform were also employed to probe their effect on the monolayer characteristics.
Much progress has been made to characterize thousands of proteins qualitatively and quantitatively in complex biological systems by use of multi-dimensional sample fractionation strategies, MS and protein micro-arrays.
Taking advantages of the efficient SAX-based and high-pH RP-based fractionation strategies, we expect that the 3D-SISPROT can be applied for the deep proteome profiling with limited starting material.
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