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Subsequently, lipids were extracted and separated in a neutral and phospholipid fraction by fractionation on a silica-G column as described [ 32].
PS1 complexes were further characterized in lumbar CSF samples and dodecylmaltoside-solubilized brain membrane fractions by fractionation on sucrose density gradients containing the detergent Brij 97.
These 9 pooled fractions were then separated by reverse phase fractionation on a Poros R2 column (4.6×50 mm, Applied Biosystems).
Purified mitochondria were prepared from a 10,000×g pellet fraction of COLO 205 cell lysates by fractionation on a continuous iodixanol gradient (19 27%).
The sRNA fraction of 19-28 nt was purified by size fractionation on a 15% TBE-Urea polyacrylamide gel.
To equalize the DNA content in different samples after fractionation on nitrocellulose, an aliquot of the F fraction was diluted 5 times, and aliquots of the R1 and R2 fractions were diluted twice.
To address this, we performed subcellular fractionation on P56 mouse brain tissue to isolate synaptosome fractions according to the methods of Dunkley, Jarvie and Robinson [ 6].
The RP fractions which were active in the bioassays were selected for further fractionation on an NP column, separating the compounds according to their polarity.
Fractionation on such gradient results in localization of the proteasome subunits in characteristic fractions [34].
This should avoid serious problems (i.e., mass fractionation) on the isotopic distributions in the original sample.
The influences of aeration rate and biodegradability fractionation on biodegradation kinetics during composting were studied.
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