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Further fractionation of the L fraction using an OptiPrep gradient showed co-fractionation of IL-1β with LC3-II.
Figure 2 shows the seven fractions obtained by RP-HPLC fractionation of the initial two solid-phase extracts.
Assay-guided fractionation of the active extract and fractions was then conducted to identify active principles.
Validation of these results were done by Western blotting after proper fractionation of the cytoplasmic and nulear fractions.
CPC fractionation of the licorice root extract resulted in 51 fractions (Fig. 1).
Size fractionation of the synthesized cDNA's was performed and 12 fractions were collected and precipitated with 100% ethanol.
Consistent with the fractionation of the siRISC, a 5′RACE product was only detected in fractions 4 6.
The dose per fraction D/N is used in the quadratic term to allow correction for fractionation of the therapy.
The polar fractionation of the acid-soluble portion clearly separates the sample into two distinct fractions.
Fractionation of the total OMC into proteins, lipids, lipoglycans, and oligo-/poly-saccharide fractions verified the observed quantitative differences.
Albertsson, P. A., Andreasson, E., Stefansson, H. & Wollenberger, L. Fractionation of the thylakoid membrane.
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