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Fractionation of samples with variation of the branching degree and end-functionalities led to series of narrowly distributed molar masses.
These might include pre-processing or fractionation of samples prior to analysis, formation of monolayers for cell interaction studies, or the enhancement of biosensor detection capability.
It is relevant to mention that the different procedures for extraction, cleanup, pre-concentration and fractionation of samples, already extensively reviewed elsewhere [3,4,60], also involve the use of different solvents and chemicals.
This is particularly evident for the thylakoid membrane isolation and fractionation of samples obtained from cultures grown under 12 hour light/dark conditions in conjunction with SCX-fractionation (Table 1).
Consequently methods using a preliminary fractionation of samples, procedure which simplifies the analysis and shortens its duration, have been developed.
To determine sarcolemmal and non-sarcolemmal Cx43 concentrations, fractionation of samples was performed as described [ 15, 19].
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Fractionation of heart samples demonstrated significantly reduced Cx43 abundance in the sarcolemma-enriched fraction isolated from paced hearts compared with sham-paced controls (40.4 ± 8.7% decrease in the paced hearts; p < 0.05; n = 11 sham and 12 paced hearts; Figure 5A).
Study on the fractionation of synthetic samples has demonstrated the applicability of the method to the preparative separation and purification of polydisperse materials.
Polarity-based fractionation of the samples was carried out through sequential solid phase extractions.
We present results for sequencing of cytoplasmic and nuclear RNA after cellular fractionation of tissue samples.
Fractionation of plasma samples and quantification of cholesterol were performed at the University of Cincinnati Mouse Metabolic Phenotyping Center.
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