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Cytosolic fractions from subcellular fractionation of control and CPT-treated HeLa cells were used to immunoprecipitate Cc.
Chromatin fractionation of control and drug-treated cells showed no evidence for an effect of the treatment in bulk cohesin recruitment (Figure 5B).
Subcellular fractionation of control versus PINK1 shRNA cells were analyzed by western blotting and show mitochondrial recruitment of endogenous Drp1 is not influenced by the loss of PINK1 (Fig. 7C).
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DOI: http://dx.doi.org/10.7554/eLife.10782.008 10.7554/eLiFigure82.009 Figure 3 figure supplement 2. Polyribosome fractionation traces of control and duplication subjects.
Further subcellular fractionation showed that PS1 was localized mainly in the synaptophysin-enriched presynaptic fractions of control mice.
The same preparations were also immunoblotted with antibodies recognizing thioredoxin (TrxA) [18], a cytoplasmic protein, to control protoplast stability, and ClfB [19], to control fractionation of the subcellular fractions.
Isotopic fractionation of boron is controlled by the exchange reactions of the boron species B(OH 3 and [B(OH 4]−.
This strongly indicates that carbon isotope fractionation of hydrocarbons is controlled by their formation processes and kinetics, which in turn may differ according to temperature, pressure, and redox conditions (McCollom and Seewald, 2006; Fu et al., 2011).
As a positive control for fractionation of genic DNA into low-copy fractions, 26 EST contigs encoding pine WRKY transcription factors were extracted from the Plant Transcription Factor Database (http://planttfdb.cbi.pku.edu.cn) and used as queries to interrogate the trimmed datasets.
For the subcellular fractionation of proteins, HPRT-knockdown and control SH-SY5Y cells and LND patient fibroblast cells were lysed and fractionated by Qproteome Cell Compartment Kit (Qiagen) according to the manufacturer's instructions.
As shown in Figure 1a, tyrosine phosphorylation as well as a visible band shift of p63 was detected in SDS-PAGE fractionation of c-Abl but not control kinase assays, in an ATP-dependent manner.
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