Finally, we used cell fractionation methods to estimate the fraction of Shc molecules in both membrane and cytoplasmic pools.
In order to ascertain that Hsp27 was translocated to the nucleus, we set out to monitor the presence of Hsp27 in cytosolic and nuclear fractions obtained by using classical cellular fractionation methods.
The presence of TMD1 in the P fraction highlights the limitations of using simple fractionation methods alone to resolve the targeting of IP3R fragments to the ER.
To determine the subcellular distribution of STIM1, we fractionated rat brain by differential and density gradient fractionation methods as previously described [25], [26].
Salmonella-based assays (some miniaturized) have been coupled with fractionation methods for most studies aimed at identifying mutagenic fractions and chemical classes in mixtures.
Testing different approaches to quantify the dissolved fraction of a Ag ENP suspension, five off-line fractionation methods as well as measurements with an ISE were compared; the results (mean value ± CI; α = 0.05) are presented in Fig. 3.
This step occurs in the periplasm [37], but our results are not in disagreement with this because the physical fractionation methods used do not allow to distinguish periplasmic and cytosoluble fractions in Brucella.
von Lützow M, Kögel-Knabner I, Ekschmitt K, Flessa H, Guggenberger G, Matzner E, et al. SOM fractionation methods: Relevance to functional pools and to stabilization mechanisms.
Helfrich, M., Flessa, H., Mikutta, R., Dreves, A. & Ludwig, B. Comparison of chemical fractionation methods for isolating stable soil organic carbon pools.
Chen, X., Wu, D., Zhao, Y., Wong, B.H. & Guo, L. Increasing phosphoproteome coverage and identification of phosphorylation motifs through combination of different HPLC fractionation methods.
A variety of biochemical fractionation methods are available for the quantification of cytoskeletal components.
